DNA

Part:BBa_K4737007:Design

Designed by: Jintao Qiu   Group: iGEM23_BNUZH-China   (2023-08-25)


pLtetO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected promoter pLtetO-1 which can be repressed by tetR for our experiment.

Source

The source of the part will be its sequence which was retrieved from GenBank.

References

Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. doi: 10.1093/nar/25.6.1203. PMID: 9092630; PMCID: PMC146584.